This is the first comparative study using GPC-LS to provide information of European hake and Blue shark collagen subunit composition. The electrophoretic patterns and gel permeation chromatography with light-scattering detection (GPC-LS) results of the obtained cation exchange peak fractions revealed differences regarding subunit composition between both species, influencing the crosslinking pattern. To test if the different chromatographic behavior exhibited by both collagens could be influenced by differences in subunit composition (alpha-chains), cation exchange chromatography was employed to separate collagen subunits. Conversely, the application of temperature and stirring-time increments showed a positive effect on Blue shark collagen solubility, resulting in different chromatographic profiles and observing higher molecular weight components when sample is incubated at 50 ☌ (15 min) after 48 h stirring. Increases of temperature and stirring time produced no effect on European hake collagen solubility in the mobile phase, resulting in the same chromatographic profiles. Chromatographic behavior revealed differences in collagen from both species. As the structure of collagen determines its function, the final objective of this study was to investigate biochemical differences between both collagens to identify future potential applications. Based in California’s majestic central coast, D E E P B L U E’s clients range from small business to Fortune 500, from start-ups to 100-year-old stalwarts, from non-profits to government agencies.Acid-soluble collagens from European hake and Blue shark skin were isolated, characterized, and compared. They succeed in providing their customers with a unique and engaging multi-platform experience. Their capabilities range from custom websites with responsive design and robust CMS solutions to branding and marketing campaigns, all with a deep focus on the user experience. D E E P B L U E offers a suite of creative services to improve the online visibility and impact of your business. They create impactful multi-platform solutions for meaningful brands. About D E E P B L U Eĭ E E P B L U E is a full-service, custom San Francisco web designer that specializes in creating an entirely new user experience through responsive design, placing an equal imperative on both desktop and mobile devices. It is collaboration between the Save Our Seas Shark Center, University of Cape Town, Department of Environmental Affairs and Tourism Marine and Coastal Management Branch and Shark Spotters. The Save Our Seas Foundation White Shark Project was initiated in 2003 and is a dedicated research program focusing on the ecology and behavior of white sharks in False Bay, Cape Town, South Africa. We are eternally grateful to Save Our Seas for honoring us by naming the shark Deepblue in thanks for our support in the White Shark Conservation Project initiative.Ībout The Save Our Seas Foundation White Shark Project This was the first successful overnight continuous track of a white shark in False Bay, South Africa. The Save Our Seas Foundation White Shark Conservation Project, together with Shark Spotters, tagged a 9-foot female great white shark and tracked her for 24 hours straight in False Bay. Great White Shark Leads Scientists Around The Clock
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